Incipient cytorrhysis assay
WebNov 16, 2024 · An incipient cytorrhysis assay was carried out by allowing appressoria to form in water on borosilicate cover slips for 24 hr, after which the water was replaced with a range of aqueous glycerol ranging from 0.25 to 2.5M and, after 30 min, the frequency of cytorrhysis determined ( de Jong et al. 1997 ). WebApr 16, 2024 · The penetrating peg, with the aid of the mechanical force, ruptures the leaf cuticle and epidermal cell wall and differentiates subsequently into invasive hyphae that ramify within the host cell and spread from cell to cell, resulting in visible lesions and rice blast symptoms [ 2 ].
Incipient cytorrhysis assay
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WebJan 26, 2016 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5 M glycerol solution as previously described (Foster et al. 2003). The onion penetration assay was performed as previously described (Wang et al. 2013). Onion epidermal strips were isolated and 40 μl of conidial suspension was inoculated onto the adaxial surface.
WebJan 26, 2024 · To investigate whether the reduced pathogenicity of ΔMopcs60 was associated with appressorium-mediated penetration, we first determined the rate of … WebApr 7, 2024 · Appressorium turgor pressure was analyzed by the incipient cytorrhysis assay using 1–4 M of glycerol solution, as described by Wang et al. (2024). The experiment was conducted three times with at least 100 structures per replicate. Onion penetration assays were conducted as described by Wang et al. (2024).
WebOct 3, 2024 · Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross … WebAug 20, 2024 · To elucidate the defect in appressorium penetration caused by myriocin/FB1, we measured the internal turgor pressure within appressoria using an …
WebAug 29, 2016 · Appressoria formed at 24 h post-incubation were used in an incipient cytorrhysis assay to measure appressorial turgor. The experiments were replicated three times and more than 200 appressoria were counted for …
WebDec 22, 2006 · Appressorial Cytorrhysis Assay in Wild-Type Guy11 and abc3 ... that the Abc3-GFP protein was highly expressed and concentrated in the appressorial plasma membrane right from the incipient stage onwards . However, at a later stage (17 h after germination) during ... binchanWebFeb 9, 2012 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–5 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspension (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature. bin chartsWebJul 1, 2024 · Appressorium turgor was measured following the incipient cytorrhysis (cell collapse) assays ( Howard et al., 1991 ). The plastic coverslips were prepared to incubate droplets (20 μl) of conidial suspension (1 × 10 5 conidia/mL). The setup was placed in a humid chamber for 24 h. cyrus loghmanee mdWebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying … binch chatWebDec 20, 2011 · The turgor pressure exerted by a mature appressorium was measured using an incipient cytorrhysis test reported earlier ( Howard et al. 1991 ). The rate of appressoria formation on a hydrophobic surface was similar in both the wild-type strain Guy11 and the Moplaa deletion mutant, as shown in Table 2. bin chat iaWeb(A) co-IP assay. Western blot analysis with total proteins (Total) isolated from transformants co-expressing MoMIP11 - GFP and MoPDEH -3xFLAG and proteins eluted from anti-GFP beads (Elution). The presence of MoMip11 and MoPdeH was detected with the anti-GFP and anti-FLAG antibody, respectively. cyrus loghmanee websiteWebJan 26, 2024 · To investigate whether the reduced pathogenicity of ΔMopcs60 was associated with appressorium-mediated penetration, we first determined the rate of appressorium collapse in the presence of various hyperosmotic concentrations of glycerol by performing an incipient cytorrhysis assay. bin chat gpt