Cisplatin h460

Author: jekm

August undefined, 2024

WebInjection, cisplatin, powder or solution, 10 mg: BETOS Code: O1D - Chemotherapy: Action Code: N - No maintenance for this code: Type of Service Code: 1 - Medical care: Pricing … WebJul 28, 2024 · Results: Herein, role of autophagy in cisplatin resistance was indicated by a decreased cell viability and increased apoptosis in lung cancer H460 cells pre-incubated …

Cisplatin-induced hydroxyl radicals mediate pro-survival …

Web检测H460细胞中p-Akt蛋白的表达。将细胞接种于6孔板中，加入不同浓度(10、20、30 μmol/L)芝麻素处理12 h后，在每孔细胞中加入100 μL的蛋白裂解液后，采用BCA试剂盒测定蛋白浓度，计算每个泳道的上样量为70 μg总蛋白，计算5× loading buffer量及超纯水量，定 … WebMay 27, 2024 · Epithelial non-small lung cancer H460 cells (generated from a pleural effusion of a patient with large cell lung cancer) and H1299 cells (derived from an NSCLC carcinoma) were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) (Life Technologies, Grand Island, NY, USA). software gratis pengganti adobe illustrator

Resistance mechanism to cisplatin in NCI-H460 non-small cell lung ...

WebJan 26, 2012 · The cisplatin resistant clone (H460/cis) of H460 cells was established by exposing the cells with gradually increasing concentrations of cisplatin until chemoresistance acquisition was elucidated by MTT, Hoechst 33342 staining and comet assays. Degree of autophagosome formation and level of LC3 marker were evaluated by … WebApr 13, 2024 · Cisplatin‑resistant A549 (A549/CDDP) and H460 (H460/CDDP) cells were first established and were then transfected with negative control small interfering (si)RNA (si‑NC) or TRPC1 siRNA (si‑TRPC1). Cells were then treated with 740 Y‑P, a PI3K/Akt agonist. Subsequently, the sensitivity of A549/CDDP and H460/CDDP cells to CDDP … WebDec 16, 2015 · H460 lung cancer cells increased cisplatin sensitivity and showed reduced repair of both . intrastrand and interstrand adducts suggesting that the mechanism of sensitivity is primarily . slow friday berlin

The IC 50 concentrations detected in A549 and H460 …

Cypripedin, a phenanthrenequinone from Dendrobium …

WebJul 28, 2024 · Cisplatin-induced cytotoxicity in human lung cancer cells. a MTT assay revealed the significant reduction in the relative cell viability in lung cancer H460 cells after treatment with 10–100 µM cisplatin for 24 h. b Increased level of apoptosis was noted after culturing with cisplatin at 25–100 µM. c Bright blue fluorescence of Hoechst33342, … WebApr 14, 2024 · To verify the resistance of A549/CR and H460/CR cells, the effect of cisplatin or PGG on viability (Figure 1A) and the PTEN expression level was examined in A549 and H460 parental cells and cisplatin-resistant A549/CR and H460/CR cells using an MTT assay and Western blotting. Cisplatin-resistant cell lines demonstrated a subtle … software gratis iso 9001 2015WebNov 5, 2024 · Oroxylin A attenuates hypoxia-induced cisplatin resistance a H460 cells were treated with cisplatin under normoxic (20% O2) or hypoxic (1% O2) condition for 36 h. The cell viability was analyzed ... slow french translation

"WebJul 26, 2024 · Expression profiling of cisplatin resistant cells derived from H460 lung cell line. Organism: Homo sapiens: Experiment type: Expression profiling by array: … " - Cisplatin h460

Cisplatin h460

Cisplatin induces A549 and H460 cell apoptosis via the.

WebLung cancer is a malignant tumor characterized by a rapid proliferation rate, less survivability, high mortality, and metastatic potential. This review focuses on updated research about the clinical application of traditional Chinese medicine (TCM) as an adjuvant therapy to lung cancer treatment and the mechanisms of TCM effect on lung cancer in … WebJan 26, 2024 · Emodin enhances cisplatin-induced DNA damage in A549 and H460 cells Cisplatin mainly kills tumor cells by inducing DNA damage ( 30 ). The effect of emodin on the chemosensitivity of A549 and H460 …

Did you know?

WebJan 17, 2013 · Cisplatin concentrations (IC 50) varied between all four cell lines (A549 5.95 µM, SKMES-1 2.65 µM, MOR 3.3 µM, H460 5.0 µM) and were subsequently used to treat each parent cell line in order to generate corresponding age and passage-matched cisplatin resistant cell lines. In the case of H460 cells, maintenance of the resistant subline was ... WebMay 11, 2012 · Cisplatin ( cis -diamminedichloroplatinum) is a platinum-based agent, which has been wildly utilized in systemic therapeutic regimens for lung cancer, both NSCLC and small-cell lung cancer. 5...

WebSep 1, 2006 · Figure 2C shows that H460-pMEP 4-MKP-1 cells treated with CdSO 4 were more resistant to both doses of cisplatin compared with the control H460-pMEP 4 vector cells . Thus, these results indicate that overexpression of MKP-1 plays a critical role in cisplatin resistance in the H460 human lung cancer cell. WebCISPLATIN 10 MG INJECTION: Short descriptive text of procedure or modifier code (28 characters or less). The AMA owns the copyright on the CPT codes and descriptions; …

WebResistance mechanism to cisplatin in NCI-H460 non-small cell lung cancer cell line: investigating apoptosis, autophagy, and cytogenetic damage … WebJul 6, 2024 · A2780 and H460 cells were treated with increasing concentrations of cisplatin (Hospira, Australia) for 48 h, collected, washed twice by centrifugation at 900 g for 5 min with FACS buffer (2.5 % FCS, 0.04 % sodium azide in PBS) and incubated with 5 μg/mL chDAB4 or human IgG (Intragram P; as control) for 30 min. Cells were washed and …

Web用 BA 对 H460 细胞进行处理，采用 MTT 法检测细胞增殖情况，并利用 Annexin V-FITC/PI 双染法检测 BA 对 H460 细胞凋亡的影响。Hoechst 33342 染色和免疫荧光染色观察细胞凋亡时染色质的凝集以及 DNA 损伤标志蛋白 γ-H2AX 表达情况。

WebJul 28, 2024 · Cisplatin induces an autophagic response in lung cancer H460 cells. To investigate the autophagic response, the alteration of autophagy marker proteins was … slow french songsWebFeb 4, 2024 · H460 cells showed a greater sensitivity to cisplatin with 41% inhibition of colony formation at 1 µM cisplatin and 80% inhibition at 2 µM, compared with 23% and 32%, respectively, for A549... slow frequency hopping refers toWebFeb 9, 2024 · Apoptotic induction of cisplatin in NSCLC H460 cells. The accumulative study indicated that the aberrant expressions of the apoptotic Bcl-2 family proteins play a central role in resistance to cisplatin. Prior to testing the apoptosis-potentiating effect of CYP, we characterized the cell death profile in response to cisplatin. ... software gratis para inventariosWebApr 10, 2024 · Microarray data from resistant and sensitive cisplatin H460 lung cancer cell line: Data processing: The analysis and visualization of the microarray data was performed using Agilent Cytogenomic 3.0.1.1. Submission date: Apr 11, 2024: Last update date: Apr 10, 2024: Contact name: Carlos Rodriguez Antolin: Organization name software gratis itbWebSep 15, 2024 · Cisplatin is a prescription medicine used to treat the symptoms of cancer in the testicles (Metastatic Testicular Tumors), bladder (Advanced Bladder Cancer) and … software gratis per disegnareWebMay 28, 2024 · Cells endogenously expressing high (A549 and H460 cells) versus low (EBC-1, CRL-5889 and HTB-182 cells) CDCA3 levels were untreated, cisplatin treated for 12 h (cisplatin) or cultured in... slow fridge waterWebApr 9, 2024 · However, in the H460 cell line with the low basal expression level of SNRPB, overexpressing of SNRPB markedly increased cisplatin-induced cell proliferation … software gratis per creare siti web